كابينة العزل الجرثومي

Enable JavaScript and cookies to continue.

Enter the email address you signed up with and we39;ll email you a reset link.Sorry, preview is currently unavailable. You can download the paper by clicking the button above..

this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 106 acinar cells can be obtained from a single murine pancreas. This protocol offers the possibility to independently process as many as 10 pancreases in parallel. Because it preserves acinar architecture, this model is well suited for studying the physiology of the exocrine pancreas in vitro in contrast to cell lines established from pancreatic tumors, which display many gic alterations resulting in partial or total loss of their acinar differentiation..

Vaccines are a 20th century medical marvel. They have dramatically reduced the morbidity and mortality caused by infectious diseases and contributed to a striking increase in life expectancy around the globe. Nheless, determining vaccine efficacy remains a challenge. Emerging evidence suggests that the current acellular vaccine (aPV) for Bordetella pertussis (B. pertussis) induces suboptimal immunity. Therefore, a major challenge is designing a next-generation vaccine that induces protective immunity without the adverse side effects of a whole-cell vaccine (wPV). Here we describe a protocol that we used to test the efficacy of a promising, novel adjuvant that skews immune responses to a protective Th1/Th17 phenotype and promotes a better clearance of a B..

Dry root rot (DRR) disease is an emerging biotic stress threat to chickpea cultivation around the world. It is caused by a soil-borne fungal pathogen, Rhizoctonia bataticola. the literature,prehensive and detailed step-by-step protocols on disease assays are sparse. This article providesplete details on the steps involved in setting up a blotting paper technique for quickly screening genotypes for resistance to DRR. The blotting paper technique is easy and less expensive. Another method, based on the sick pot approach, is a mimic of natural infection and can be applied to study the interactingponents8212;plant, pathogen, and environment8212;involved in the disease triangle..

The human blood-brain barrier selectively prevents pration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial traversal.The human blood-brain barrier (BBB) is characterized by a very low permeability for biomolecules in order to protect and regulate the metabolism of the brain. The BBB is mainly formed out of endothelial cells embedded in collagen IV and fibronectin-rich basement membranes. Several pathologies result from dysfunction of the BBB followed by microbial traversal, causing diseases such as meningitis..

This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.Several protocols have been reported in the literature for the isolation and culture of human amniotic epithelial cells (HAEC). However, these assume that the amniotic epithelium is a homogeneous layer. The human amnion can be divided into three anatomical regions: reflected, placental, and umbilical. Each region has different physiological roles, such as in pathological conditions. Here, we describe a protocol to dissect human amnion tissue in three sections and maintain it in vitro..

Enable JavaScript and cookies to continue.

lt;iframe src=” width=”600″ height=”497″ frameborder=”0″ marginwidth=”0″ marginheight=”0″ scrolling=”no” style=”border:1px solid CCC;border-width:1px 1px;margin-bottom:5px;max-width: 100;” allowfullscreen webkitallowfullscreen mozallowfullscreengt; lt;/iframegt;2- تشخيص أمراض النبات البكتيرية. من الثابت أنه لا يمكن الاعتماد على الأعراض الظاهرية كوسيلة لتشخيص المرض وذلك لتشابه الأعراض . فعلى سبيل المثال تتشابه أعراض العفن الطري البكتيري والعفن الطري الفطري . وتتشابه التدرنات البكتيرية Bacterial Galls والحشرية sect Galls.2- تشخيص أمراض النبات البكتيرية • من الثابت أنه لا يمكن الاعتماد على الأعراض الظاهرية كوسيلة لتشخيص المرض وذلك لتشابه الأعراض. فعلى سبيل المثال تتشابه أعراض العفن الطري البكتيري والعفن الطري الفطري. وتتشابه التدرنات البكتيرية Bacterial Galls والحشرية sect Galls . • ومن ناحية أخرى نجد أن العفن الطري البكتيري قد تسببه أجناس من البكتيريا Erwinia أو من البكتيريا Pseudomonas ..

Atomic Force Microscopy-frared Spectroscopy (AFM-IR) provides a powerful platform for bacterial studies, enabling to achieve nanoscale resolution. Both, mapping of subcellular changes (e.g., upon cell division) as well asparative studies of chemicalposition (e.g., arising from drug resistance) can be conducted at a single cell level in bacteria.Atomic Force Microscopy-frared Spectroscopy (AFM-IR) is a novelbinatory technique, enabling simultaneous characterization of physical properties and chemicalposition of sample with nanoscale resolution. bining AFM with IR, the spatial resolution limitation of conventional IR is ovee, enabling a resolution of 208211;100 nm to be achieved. This opens the door for a broad array of new applications of IR toward probing samples smaller than several micrometers, previously unachievable by means of conventional IR microscopy..

Pseudomonas aeruginosa, an opportunistic Gram-negative bacterial pathogen, can overproduce an exopolysaccharide alginate resulting in a unique phenotype called mucoidy. Alginate is linked to chronic lung infections resulting in poor prognosis in patients with cystic fibrosis (CF). Understanding the pathways that regulate the production of alginate can aid in the development of novel therapeutic strategies targeting the alginate formation. Another disease-related phenotype is the small colony variant (SCV). SCV is due to the slow growth of bacteria and often associated with increased resistance to antimicrobials. this paper, we first show a method of culturing a gically defined form of P..

The recent epidemic of Zika virus highlights the importance of establishing reverse gic approaches to develop vaccines and/or therapeutic strategies. Here, we describe the protocol to rescue an infectious rbinant Zika virus from a full-length cDNA clone assembled in a bacterial artificial chromosome under the control of the human cytomegalovirus immediate-early promoter.The association of Zika virus (ZIKV) infection with neurologicalplications during the recent worldwide outbreak and the lack of approved vaccines and/or antivirals have underscored the urgent need to develop ZIKV reverse gic systems to facilitate the study of ZIKV biology and the development of therapeutic and/or prophylactic approaches. However, like with other flaviviruses, the generation of ZIKV full-length infectious cDNA clones has been hampered due to the toxicity of viral sequences during its amplification in bacteria..

Here we present a protocol for the rapid identification of proteins produced by genomically sequenced pathogenic bacteria using MALDI-TOF-TOF tandem mass spectrometry and top-down proteomic analysis with software developed in-house. Metastable protein ions fragment because of the aspartic acid effect and this specificity is exploited for protein identification.This protocol identifies the immunity proteins of the bactericidal enzymes: colicin E3 and bacteriocin, produced by a pathogenic Escherichia coli strain using antibiotic induction, and identified by MALDI-TOF-TOF tandem mass spectrometry and top-down proteomic analysis with software developed in-house. The immunity protein of colicin E3 (Im3) and the immunity protein of bacteriocin (Im-Bac) were identified from prominent b- and/or y-type fragment ions generated by the polypeptide backbone cleavage (PBC) on the C-terminal side of aspartic acid, glutamic acid, and asparagine residues by the aspartic acid effect fragmentation mechanism..